Everyday impunity in Myanmar, lessons from Bangladesh

Conditions in Myanmar have changed sufficiently over the last few years to enable people to challenge everyday impunity in a way that would not previously have been possible. Nevertheless, Nick Cheesman draws on the case of Bangladesh to point to obstacles to aggrieved persons seeking redress for state violence. The continued political and economic power of Myanmar’s armed forces coupled with a corresponding absence of institutionalised legal avenues mean long-term structural changes will be key to successfully redressing grievances against state officers

Tyr25, Tyr58 and Trp133 of Escherichia coli bacterioferritin transfer electrons between iron in the central cavity and the ferroxidase centre

Ferritins are 24meric proteins that overcome problems of toxicity, insolubility and poor bioavailability of iron in all types of cells by storing it in the form of a ferric mineral within their central cavities. In the bacterioferritin (BFR) from Escherichia coli iron mineralization kinetics have been shown to be dependent on an intra-subunit catalytic diiron cofactor site (the ferroxidase centre), three closely located aromatic residues and an inner surface iron site. One of the aromatic residues, Tyr25, is the site of formation of a transient radical, but the roles of the other two residues, Tyr58 and Trp133, are unknown. Here we show that these residues are important for the rates of formation and decay of the Tyr25 radical and decay of a secondary radical observed during Tyr25 radical decay. The data support a mechanism in which these aromatic residues function in electron transfer from the inner surface site to the ferroxidase centre

Mechanisms of iron- and O₂-sensing by the [4Fe-4S] cluster of the global iron regulator RirA.

RirA is a global regulator of iron homeostasis in Rhizobium and related α-proteobacteria. In its [4Fe-4S] cluster-bound form it represses iron uptake by binding to IRO Box sequences upstream of RirA-regulated genes. Under low iron and/or aerobic conditions, [4Fe-4S] RirA undergoes cluster conversion/degradation to apo-RirA, which can no longer bind IRO Box sequences. Here, we apply time-resolved mass spectrometry and electron paramagnetic resonance spectroscopy to determine how the RirA cluster senses iron and O2. The data indicate that the key iron-sensing step is the O2-independent, reversible dissociation of Fe2+ from [4Fe-4S]2+ to form [3Fe-4S]0. The dissociation constant for this process was determined as Kd = ~3 µM, which is consistent with the sensing of 'free' iron in the cytoplasm. O2-sensing occurs through enhanced cluster degradation under aerobic conditions, via O2-mediated oxidation of the [3Fe-4S]0 intermediate to form [3Fe-4S]1+. This work provides a detailed mechanistic/functional view of an iron-responsive regulator

Ferritins: furnishing proteins with iron

Ferritins are a superfamily of iron oxidation, storage and mineralization proteins found throughout the animal, plant, and microbial kingdoms. The majority of ferritins consist of 24 subunits that individually fold into 4-α-helix bundles and assemble in a highly symmetric manner to form an approximately spherical protein coat around a central cavity into which an iron-containing mineral can be formed. Channels through the coat at inter-subunit contact points facilitate passage of iron ions to and from the central cavity, and intrasubunit catalytic sites, called ferroxidase centers, drive Fe2+ oxidation and O2 reduction. Though the different members of the superfamily share a common structure, there is often little amino acid sequence identity between them. Even where there is a high degree of sequence identity between two ferritins there can be major differences in how the proteins handle iron. In this review we describe some of the important structural features of ferritins and their mineralized iron cores and examine in detail how three selected ferritins oxidise Fe2+ in order to explore the mechanistic variations that exist amongst ferritins. We suggest that the mechanistic differences reflect differing evolutionary pressures on amino acid sequences, and that these differing pressures are a consequence of different primary functions for different ferritins

Outreach:Impact on Skills and Future Careers of Postgraduate Practitioners Working with the Bristol ChemLabS Centre for Excellence in Teaching and Learning

Postgraduate engagement in delivering outreach activities is more commonplace than it once was. However, the impact on postgraduate students (typically studying for a Ph.D. degree) of participating in the delivery of these outreach activities has rarely, if ever, been recorded. The Bristol ChemLabS Outreach program has been running for ca. 17 years, and in that time, many postgraduate students have been involved (approximately 500), with around 250 typically for up to 3 years. We sought to investigate the impact of outreach engagement on postgraduate alumni who were involved in the program for over 3 years (32) and how the experiences and training of the outreach program had impacted on their careers postgraduation. Thirty of the 32 postgraduates engaged and ∼70% reported that their outreach experience had influenced their decision making on future careers. Many respondents reported that the skills and experiences gained through outreach participation had contributed to success in applying for and interviewing at their future employers. All respondents reported that outreach had helped them to develop key skills that were valued in the workplace, specifically, communication, teamwork, organizational skills, time planning, event planning, and event management. Rather than a pleasant distraction or an opportunity to supplement income, all participants noted that they felt there were many additional benefits and that this was time well spent. Outreach should not be viewed as a distraction to science research but rather an important enhancement to it provided that the program is well constructed and seeks to develop those delivering the outreach activities

Within-host competition does not select for virulence in malaria parasites; studies with Plasmodium yoelii

In endemic areas with high transmission intensities, malaria infections are very often composed of multiple genetically distinct strains of malaria parasites. It has been hypothesised that this leads to intra-host competition, in which parasite strains compete for resources such as space and nutrients. This competition may have repercussions for the host, the parasite, and the vector in terms of disease severity, vector fitness, and parasite transmission potential and fitness. It has also been argued that within-host competition could lead to selection for more virulent parasites. Here we use the rodent malaria parasite Plasmodium yoelii to assess the consequences of mixed strain infections on disease severity and parasite fitness. Three isogenic strains with dramatically different growth rates (and hence virulence) were maintained in mice in single infections or in mixed strain infections with a genetically distinct strain. We compared the virulence (defined as harm to the mammalian host) of mixed strain infections with that of single infections, and assessed whether competition impacted on parasite fitness, assessed by transmission potential. We found that mixed infections were associated with a higher degree of disease severity and a prolonged infection time. In the mixed infections, the strain with the slower growth rate was often responsible for the competitive exclusion of the faster growing strain, presumably through host immune-mediated mechanisms. Importantly, and in contrast to previous work conducted with Plasmodium chabaudi, we found no correlation between parasite virulence and transmission potential to mosquitoes, suggesting that within-host competition would not drive the evolution of parasite virulence in P. yoelii

Reading paperwork realistically

According to Steven Lubet, documentation freezes facts in time. For this reason, it is, he thinks, more reliable than other kinds of evidence. That makes it especially useful for fact checking. However, this is not a realistic way to read for evidence in documents. It ignores or downplays a crucial fact that inheres to all paperwork, which ethnographers have long heeded: documents do not report on facts; they render them. Ethnographically informed readings of paperwork that attend both to facts and to how they are rendered are, by contrast, realistic. In this contribution, I discuss why and illustrate with reference to two studies of policing and law in Thailand

Introduction: Interpreting Communal Violence in Myanmar

Collective violence wracked Myanmar from 2012 to 2014. Overwhelmingly, Buddhists attacked Muslims. This article categorises the violence as “communal,” in so far as it consisted of recurrent, sporadic, direct physical hostility realised through repeated public expressions that Muslims constitute an existential threat to Buddhists. It advocates for interpretive modes of inquiry into the violence, as well as into the practices of interpretation enabling it. Eschewing methods aimed at producing a purportedly coherent picture of what happened, interpretive research raises questions about conventional readings of violence, and seemingly self-evident categories for its analysis. But as the articles in this special issue show, interpretivists do not repudiate the search for factual truth. The contributors all make strong truth claims, but claims recognising that factual truths are always contingent. They establish these claims by attending variously to the processes, narratives, histories and typologies that have contributed to the production of communal violence in Myanmar.The editor of this special issue acknowledges the ANU College of Asia and the Pacific for the allocation of funds from a grant provided by AusAID [Grant Agreement 65115] for the holding of the research colloquium on communal conflict in Myanmar on March 17 and 18, 2014

Interpreting Communal Violence in Myanmar

Myanmar's recovery from half a century of military rule has been fraught. As in other religiously, culturally and linguistically heterogeneous countries where a dictatorship has loosened a tight grip, people there have wanted for democratic institutions to express and manage conflict. Under these circumstances, mundane and seemingly apolitical events sometimes unfold into moments of intense violence. Interpreting Communal Violence in Myanmar addresses one such violent chapter in Myanmar’s recent past: the communal violence that shook the country between 2012 and 2014. The violence, most of it involving Buddhists attacking Muslims, ranged from localised, fleeting, inter-group melees, to large scale, apparently well-organised, state-supported killing and destruction of property of a targeted community, running over a number of days. The book’s seven chapters comprise a response to the violence by a group of Myanmar and Southeast Asia experts. Their contributions trace the histories and contemporary features of the violence, and the legal and political arrangements that made it possible. Their interpretations, while specific to Myanmar, also contribute to broader debate about the characteristics, causes and consequences of communal violence generally. The chapters were originally published as a special issue in the Journal of Contemporary Asia